Da fatan za a yi zaɓinku bisa yanayin dakin gwaje-gwajenku.
Yana da mahimmanci don nemo hanya mai mahimmanci don tsaftace samfurin samfurin
A halin yanzu, akwai adadi mai yawa na samfuran kayan aikin gona (sauran samfuran sinadarai, Organic acid, da sauransu) waɗanda ke buƙatar gwada ruwa chromatography da gas chromatography kowace shekara.Saboda yawan samfurori, akwai adadi mai yawa na samfurin samfurin da ake buƙatar tsaftacewa yayin aikin ganowa, wanda ba wai kawai ɓata lokaci ba ne kuma yana rage aikin aiki, amma har ma a wasu lokuta yana haifar da sabani a cikin sakamakon gwaji saboda tsaftacewa. da tsabtace samfurin vials.
Filayen samfurin chromatographic galibi an yi su ne da gilashi, ba safai ake yin su da filastik ba.Filayen samfurin da za a iya zubarwa suna da tsada, ɓatacce, kuma suna ƙazantar da muhalli.Yawancin dakunan gwaje-gwaje suna tsaftace samfurin kwalabe da sake amfani da su.
A halin yanzu, hanyoyin da aka saba amfani da su na dakin gwaje-gwaje ana yin wankin bututu galibi ana ƙara wanki, wanki, abubuwan kaushi da kuma wankin acid, sannan ƙayyadaddun tsarin goge ƙananan bututu.
Wannan hanyar gogewa ta al'ada tana da illoli da yawa:
Yin amfani da wanka yana cinye ruwa da yawa, lokacin wankewa yana da tsawo, kuma akwai sasanninta da wuya a tsaftace.Idan kwalayen samfurin filastik ne, yana da sauƙi a sami alamun buroshi a cikin bangon vials, wanda ke ɗaukar albarkatun aiki da yawa.Don kayan gilashin da ke da gurɓataccen gurɓataccen ƙwayar lipid da furotin, ana amfani da maganin alkaline lysis don tsaftacewa, kuma ana samun sakamako mai kyau.
Lokacin nazarin samfurori ta LC/MS/MS, tsaftacewa na alluran allura yana da mahimmanci.Bisa ga hanyar tsaftacewa na gilashin gilashi, an zaɓi hanyar tsaftacewa bisa ga girman ƙazanta.Babu ƙayyadadden yanayi.Takaitacciyar hanyar:
Zabin Daya:
1. Zuba maganin gwajin a cikin busassun busassun
2. Dip duk gwajin gwajin a cikin barasa 95%, wanke shi sau biyu tare da ultrasonic kuma zubar da shi, saboda barasa cikin sauƙi ya shiga cikin 1.5mL vial kuma zai iya zama miscible tare da mafi yawan kaushi na kwayoyin don cimma sakamako mai tsabta.
3. Zuba cikin ruwa mai tsabta, kuma ultrasonically wanke sau biyu.
4. Zuba ruwan shafa a cikin busassun busassun kuma gasa a 110 digiri Celsius na 1 zuwa 2 hours.Kada a taɓa yin gasa a yanayin zafi mai yawa.
5. Cool da ajiyewa.
Zabi na biyu:
1. Kurkura da ruwan famfo sau da yawa
2. Saka shi a cikin kwano mai cike da ruwa mai tsabta (Millipore pure water machine) da sonicate na minti 15.
3. Canja ruwa da duban dan tayi na minti 15
4. Jiƙa a cikin beaker cike da cikakken ethanol (Rukunin Sinopharm, Tsabtace Tsabta)
5. A ƙarshe, fitar da shi kuma bar shi ya bushe.
Zabi na uku:
1. Na farko jiƙa a methanol (chromatographically tsarki), da kuma ultrasonically tsabta na minti 20, sa'an nan zuba methanol bushe.
2. Cika samfurin vials da ruwa, kuma ultrasonically tsabta don minti 20, zuba ruwan .
3. Bushe samfurin vials daga baya.
Zabin Hudu:
Hanyar wankewar samfurin samfurin daidai yake da shirye-shiryen lokacin ruwa, da dai sauransu. Da farko, yi amfani da barasa na likita don jiƙa fiye da sa'o'i 4, sa'an nan kuma duban dan tayi na rabin sa'a, sannan a zubar da barasa na likita, kuma a yi amfani da ruwa. don rabin duban dan tayi.Awanni, kurkura da ruwa kuma bushe shi.
Zabi na biyar:
Na farko, jiƙa a cikin wani karfi mai tsabta tsaftacewa (potassium dichromate) na tsawon sa'o'i 24, sa'an nan kuma amfani da ruwa mai tsabta a cikin ultrasonic A wanke shi sau uku a karkashin yanayi, kuma a karshe wanke shi da methanol sau ɗaya, sa'an nan kuma bushe shi don amfani.
Dole ne a maye gurbin septas na caps, musamman lokacin nazarin ragowar magungunan kashe qwari, in ba haka ba zai shafi sakamakon ƙididdiga.
Amma idan yanayi ya ba da izini, gwada amfani da abubuwan da za a iya zubar da su, kamar abubuwan da za a iya zubar da su na PTFE ko abubuwan da ake sakawa na filastik na cikin gida (kimanin yuan 0.1 / yanki), kuma samfurin filaye suna da kyau.Maimaita amfani kuma baya buƙatar tsaftacewa.
Zabi na shida:
(1) rikitarwa tsarin tsaftacewa tare da sakamako mai amfani:
No1.Bayan an yi amfani da filayen samfurin, kurkura samfurin samfurin tare da ruwa mai gudu da farko, kuma ku wanke sauran samfurin (zaku iya girgiza shi da hannu a lokaci guda);
No2, sai a zuba samfurin samfurin a cikin kumfa na potassium dichromate na wanke ruwa, idan ya taru idan ya kai wani adadi ko kuma lokacin da kake cikin yanayi mai kyau, cire shi daga cikin tankin ruwan shafa a saka a cikin sieve filastik don dafa abinci. amfani.Kurkura sosai da ruwan famfo.Kuna iya sake zazzagewa da girgiza a tsakiya;
No3.Yi amfani da ruwan famfo don tsabtace ultrasonically sau 3 bayan kurkura.A kusa da, ya fi dacewa don girgiza ruwa a cikin samfurin samfurin bayan kowane tsaftacewa na ultrasonic;
No4, sannan yi amfani da ruwa mai sau uku (ko ruwa mai tsabta, ruwa mai tsabta) tare da tsaftacewa na ultrasonic 1.3 sau uku;
No5, to, yi amfani da chromatographic tsarki methanol ultrasonic tsaftacewa 2-3 sau, shi ne kuma mafi kyau ga
girgiza methanol daga cikin samfurin samfurin bayan kowane tsaftacewa;
No6.Saka filayen samfurin a cikin tanda kuma bushe shi a kusan digiri 80, kuma ana iya amfani dashi.
(2) samfurin vials da aka saya don yin alama da launuka daban-daban:
idan kun lura cewa akwai ƙaramin alama mai launi a kan samfuran samfuran, wanda ba don kyan gani ba, amma yana da amfani.Lokacin siyan, ya fi dacewa don siyan vials da yawa na launuka daban-daban.
Misali: dakin gwaje-gwajenku yana buɗe ayyuka biyu A da B a lokaci guda.A karo na farko A aikin yana amfani da farar samfurin vials, kuma aikin B yana amfani da buɗaɗɗen samfurin samfurin.Bayan an gama gwajin, ana tsaftace shi bisa ga hanyar da aka ambata a sama, kuma gwaji na biyu A lokacin, yi amfani da samfurin samfurin blue don aikin A, farar samfurin samfurin B, da sauransu, wanda zai iya guje wa matsalolin da ke haifar da su yadda ya kamata. gurbacewar aikinku.
Rubuta a karshen
1. Injiniyoyin kayan aiki da yawa sun ba da shawarar: Yi amfani da tanderun murfi a digiri 400 don yin gasa na rabin sa'a, abubuwan halitta sun ɓace;
2. Saka filayen samfurin a cikin tanderun murfi don bushewa a 300 digiri Celsius.Wani injiniyan Agilent daga birnin Beijing ya ce lokacin da ya zo wurin murhu, gwajin ba zai zama hayaniya ba bayan yin burodi a cikin tanderun da ke da digiri 300 na sa'o'i 6.
kuma………..
Ana iya tsaftace ƙananan filayen volumetric, filaye masu siffar pear don ƙafewar juyawa, da sauran kayan gilashin don bincike ko riga-kafi za a iya tsabtace su ta hanyar komawa zuwa wannan hanyar.
Lokacin aikawa: Fabrairu-25-2022